Journal: Open Life Sciences
Article Title: Circ_0091579 enhances the malignancy of hepatocellular carcinoma via miR-1287/PDK2 axis
doi: 10.1515/biol-2021-0009
Figure Lengend Snippet: Circ_0091579 overexpression has no influence on the proliferation and apoptosis of THLE-2 cells. THLE-2 cells were transfected with pLCDH-ciR empty vector or circ_0091579 plasmid. (a) MTT assay was performed to generate cell proliferation curve by analyzing the number of THLE-2 cells after transfection for 24, 48, or 72 h. (b) Flow cytometry was conducted to evaluate the apoptosis rate in transfected THLE-2 cells.
Article Snippet: Empty vector (pLCDH-ciR) and circ_0091579 overexpression plasmid (circ_0091579) were purchased from Ribobio (Guangzhou, China).
Techniques: Over Expression, Transfection, Plasmid Preparation, MTT Assay, Flow Cytometry